Detection of DNA Heteroduplices by Denaturing High Performance Liquid Chromatography Stanford Reference: S95-024 Abstract Stanford researchers have developed a chromatographic method for separating heteroduplex and homoduplex nucleic acid fragments. After melting and cooling fragments of double stranded DNA from diverse samples, a segment of nucleic acids is screened via HPLC under defined conditions. As the result, strands with any degree of mismatch will display characteristic and highly visible HPLC profiles. This technique detects single base mismatches in DNA fragments as large as 1 kilobase, offering effective analysis of paired DNA strands for applications such as forensic analysis, SNP discovery, and evaluation of gene expression. Additional Research: In the laboratories of Dr. Underhill and Dr. Oefner, they are interested in deciphering human DNA sequence variation, analyzing Y chromosome variation, and cataloging the common sequence variation associated with human genes implicated in the susceptibility, resistance or environmental influences associated with disease in DNA isolated from geographically diverse human populations. Additionally, at the Stanford DNA Sequencing and Technology Development Center they are working on DNA sequencing and developing tools to speed sequencing endeavors. For more information about this research visit http://www-sequence.stanford.edu/ For related technology (Software to Determine Optimum Temperature for DHPLC), please review Stanford Docket S97-175 at http://availtech.stanford.edu/Scripts/otl.cgi/docket?docket=97-175 Applications For initial identification of which variants This invention can be used to uncover >95% polymorphic loci in a specific genome, identifying genetic markers for purposes including linkage analysis, evolutionary studies, and forensic applications. It can also be used diagnostically to identify mutations associated with disease states. Furthermore, analysis of heteroduplexes commonly formed in competitive PCR reactions permits absolute quantitation of gene expression. Advantages This invention represents the most powerful technique developed to date to detect single nucleotide polymorphisms (SNPs). It requires minimal DNA (picomoles) and no sample preparation. Sample analysis is rapid (in minutes), reproducible, quantitative, and automatable. The material can be fully recovered for further analysis. Publications U.S. Patent No. 5,795,976 Innovators & Portfolio Peter Oefner   more technologies from Peter Oefner » Peter Underhill   more technologies from Peter Underhill » Patent Status Issued: 5,795,976 (USA) Date Released: 08/30/1997 Licensing Contact Kirsten Leute, Senior Associate kirsten.leute@stanford.edu 1-650-723-4374 S00-132   Multiplex Pyrosequencing for Typing S01-226   New Fluorescent Deoxyribosides and their Incorporation into Combinatorial Fluorophore Arrays S05-208   Thermosequencing: A Novel DNA Sequencing Method more technologies » You must be a registered user in order to request more information on technologies. If you are already registered, please login.        Recently Viewed... S95-024 Detection of DNA Heteroduplices by Denaturing High Performance Liquid Chromatography S97-248 Human Y Chromosome Specific Single Nucleotide Polymorphisms